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Fig. 5 | Orphanet Journal of Rare Diseases

Fig. 5

From: Uncovering a novel SERPING1 pathogenic variant: insights into the aggregation of C1-INH in hereditary angioedema

Fig. 5

Down-regulating GRP75, rescuing in cellular calcium overload and mitochondrial damage. (A) Validation of the efficiency of knockout for siRNA targeting GRP75. (B-C) Decreased expression of GRP75 contributes to a reduction in the release of Cytochrome C (Cyt-C). (D) Reduced GRP75 expression help to improve cell ATP synthesis recovery. (F-G) Flow cytometry was used to observe the changes in mitochondrial membrane potential (MMP). When down-regulate, the expression of GRP75 ameliorated changes in MMP. These experiments in A-E were replicated 3 times with similar results. Representative data in A-G are presented as mean ± SEM; ***, P < 0.001

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Orphanet Journal of Rare Diseases

ISSN: 1750-1172