Fig. 3

Effect pantothenate, pantethine, omega-3 and vitamin E supplementation on iron accumulation in three mutant PANK2 cells. (A) Control (C1) and three PKAN fibroblast cell lines (P1, P2 and P3) were treated with 5 µM pantothenate, 5 µM pantethine, 5 µM vitamin E and 5 µM omega 3 for 10 days (+ T). Then, cells were stained with Prussian Blue as described in Material and Methods and examined by bright-field microscopy. Scale bar = 15 µm. (B) Quantification of Prussian Blue staining (C) Iron content determined by ICP-MS. Data represent the mean ± SD of three separate experiments. Significance between PKAN and control fibroblasts is represented as ^*p < 0.01 fibroblasts and ^ap < 0.05, ^aap < 0.01 between untreated and treated fibroblasts