Fig. 5

Effect of pantothenate, pantethine, omega-3 and vitamin E supplementation on lipid peroxidation. Control and PKAN fibroblasts (P1, P2 and P3) were treated with 5 µM pantothenate, 5 µM pantethine, 5 µM vitamin E and 5 µM omega 3 for 10 days (+ T). (A) Representative images of lipid peroxidation in treated and untreated control and PKAN cells using BODIPY® 581/591 C11 staining. Control cells treated with Luperox® (500 μM) for 15 min were used as a positive control of lipid peroxidation. Scale bar = 15 μm. (B) Ratio of the oxidized BODIPY-C11 signal (green) to reduced BODIPY-C11 signal (red). Data represent the mean ± SD of three separate experiments (50 cell images for each condition). *p < 0.01 between PKAN patients and controls. ^aap < 0.01, between untreated and treated fibroblasts. A.U., arbitrary units