Genomic analysis of the SMN1 gene region in patients with clinically diagnosed spinal muscular atrophy: a retrospective observational study

Table 1 Modified primer design for amplification of SMN1

Methods	Forward primer	Reverse primer	Product length Transcript position
Original method^a	GTTGGGGGATCAAATATCTTCTAGTGTT	CCCCCACCCCAGTCTTTTACAGATGGT	28,233 bp Hg19 chr5: 70,220,277~70,248,509
Modified method	CCCATGTTTGTCTTTCCTTGTTTGTCT	CCACCCCAGTCTTTTACAGATGGTTT	28,175 bp Hg19 chr5: 70,220,332~70,248,506

bp, base pairs; chr, chromosome; hg, human genome; SMN1, survival motor neuron 1
^aAs previously described [10]

ISSN: 1750-1172