Fig. 5
Analysis of ROS levels, ATP production, and mitochondrial respiration in TK2 deletion cell. A Flow cytometry analysis of ROS levels using DCFH-DA fluorescence intensity. The graph on the right shows the quantification of DCFH-DA fluorescence, indicating significantly elevated ROS levels in the TK2 (Dele) group compared to control and TK2 (Total) groups. B Measurement of ATP production rates in the different experimental groups. The relative rate of ATP production is significantly lower in the TK2 (Dele) group compared to the control and TK2 (Total) groups, while no significant difference is observed between the control and scramble groups. C OCR analysis using the Seahorse Bioscience XFe 24 Extracellular Flux Analyzer to measure mitochondrial respiration. The graph depicts basal respiration, maximal respiration, and ATP production in the different groups. The TK2 (Dele) group shows significant reductions in all parameters compared to the control and TK2 (Total) groups. D Analysis of mitochondrial complex-specific respiration (complexes I, II, and IV) using the Seahorse Bioscience XFe 24. The graphs show reduced activity in all complexes in the TK2 (Dele) group compared to the control and TK2 (Total) groups. Statistical significance is represented as *p < 0.05, **p < 0.01, ***p < 0.001, and non-significant differences as (ns)