Co-segregation of the c.489+3A>G variant with p.Cys1400Ter pathogenic CFTR mutation in Cyprus: prevalence and clinical implications

Table 1 Primer sequences of STRs (Short Tandem Repeats) used in this study. Chromosome location observed fragment sizes and fluorescent labeling are indicated

STR Marker	Primer sequence (5’–3’)	Fluorescent Label	Chromosome location according to genome assembly GRCh38.p14	Observed sizes (bp)	Reference
D7S633	Fwd: TGAGCCTCGCATCACTG	–	117,370,884	163–181	[18]
D7S633	Rev: CTGGGGAGTCCTTTAACAGTA	VIC	117,370,752	163–181	[18]
IVS8CA	Fwd: ACTAAGATATTTGCCCATTATCAAGT	–	117,548,291	180–196	[19]
IVS8CA	Rev: AATCTATCTCATGTTAATGCTGAAGA	PET	117,548,420	180–196	[19]
IVS17BTA	Fwd: ATGCTGCATTCTATAGGTTATCAAT	–	117,611,892	199–257	[19]
IVS17BTA	Rev: GACAATCTGTGTGCATCGG	6-FAM	117,612,098	199–257	[19]
CFCA18K	Fwd: TATGAAGGAGACCAGGTCAAC	–	117,685,683	126–143	Current article
CFCA18K	Rev: CAAGGTCAGAAGTAGAGCCA	NED	117,685,782	126–143	Current article
CFAC30	Fwd: AGAATAAAGCTTGGGAGTTGC	–	117,773,639	135–162	[17]
CFAC30	Rev: CACTTCCTCTCCAACTTTGC	6-FAM	117,773,752	135–162	[17]

ISSN: 1750-1172